noxa  (Cell Signaling Technology Inc)

Journal: The FASEB Journal

Article Title: Chidamide Accelerates the Death of Senescence‐Like Diffuse Large B‐Cell Lymphoma Cells With TP53 Mutation Induced by Doxorubicin

doi: 10.1096/fj.202500962RR

Figure Lengend Snippet: CHI combined with DOX promotes apoptosis of DLBCL cells. (A) Annexin V‐FITC staining of DLBCL cells was determined by flow cytometry. DLBCL cells were treated with CHI and/or DOX for 48 h. (B) Quantitative analysis of apoptosis cells. Data represent the mean ± SEM from three independent experiments. ** p < 0.01, *** p < 0.001. (C) DLBCL cells were treated with CHI and/or DOX for 24 h. The expression of PARP‐1 and caspase‐3 were detected by western blot. (D) RI‐1 cells were pretreated with or without 50 μM Z‐VAD‐FMK for 1 h, and then treated with 2 μM CHI and 0.2 μM DOX for 24 h. The expression of PARP‐1 and caspase‐3 were detected by western blot. (E) RI‐1 cells were treated with CHI and/or DOX for 24 h. The expression of Mcl‐1, Bcl‐2, Bcl‐XL, NOXA, PUMA, Bax, and γ‐H2AX were detected by western blot. (F) DNA strand breaks in cells were tested by comet assay treated with CHI and/or DOX for 24 h. (G) Comet tail length of RI‐1 and SU‐DHL‐8 cells was used to measure DNA damage degree.

Article Snippet: Primary antibodies against β‐actin, PARP1, PUMA, and NOXA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Staining, Flow Cytometry, Expressing, Western Blot, Single Cell Gel Electrophoresis